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Home / Proteins & Peptide

3C protease, His, Human

SKU : BHP18500259

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iXCells Biotechnologies

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Mfr.No.	IX03092, IX03092-100, IX03092-250, IX03092-500
Description	Recombinant PreScission Protease (rPSP) from human rhinovirus (HRV 3C) is a recombinant restriction-grade protease. PSP is a highly purified recombinant 6xHis-fusion protein, which recognizes the same cleavage site as the native enzyme. It is used to cleave affinity tags from fusion proteins. The optimum recognition site for this enzyme is the sequence Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro(LEVLFQ/GP) and cleavage occurs between the Gln and Gly-Pro residues. The optimal temperature for cleavage is 4°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant PreScission Protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.Recombinant PreScission Protease (rPSP) contains 217 amino acids with N-terminal His tag. A fully biologically active molecule, rPSP has a molecular mass of 24 kDa and is obtained by proprietary chromatographic techniques.
Formulation	Sterile liquid solution contains 50 mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 50% Glycerol, pH 7.5.
Molecular Weight	24kDa, observed by reducing SDS-PAGE.
Source	E. coli
Host	Human
Notes	For research use only
Bioactivity	0.6 IU/ul.Unit Definition: One unit is defined as the amount of enzyme needed to cleave 100 ug of fusion protein in 16 hours to 90% completion at 4°C in a buffer containing 50 mM Tris-HCl, pH 7.0, 150 mM NaCl, 1 mM EDTA, and 1 mM DTT.

Synonyms	rPSP, PSP, HRV 3C Protease

Purity	95% by SDS-PAGE analyses.
Storage	Recombinant PreScission Protease (rPSP) remains stable up to 1 year at -20°C from date of receipt. Please avoid freeze-thaw cycles.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500259-1	IX03092-100	100IU	$49		Add to Cart
BHP18500259-2	IX03092-250	250IU	$107		Add to Cart
BHP18500259-3	IX03092-500	500IU	$174		Add to Cart

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