Protein A/G MagBeads (IP Grade)

Mfr.No.

36417ES08, 36417ES03

Description

Protein A/G MagBeads use "nano-surface biotechnology" (S-TEC) to target Protein A/G onto the surface of superparamagnetic polymer microspheres with high density. It has higher antibody binding ability and very low non-specific adsorption rate of protein. One-step purification can isolate the antibody with purity >90% from the serum sample, which is simple and effective to use. Natural Protein A is a cell wall surface protein found in Staphylococcus aureus. Natural Protein G is a cell surface protein isolated from the genus G or C Streptococcus. Both have similar functions and bind most mammalian IgG by interacting primarily with the Fc region of immunoglobulin (Ig), but differ in their binding specificity. Protein A/G MagBeads covalently conjugate both protein A and protein G, providing A wider binding range and higher utility than either protein A or protein G alone. At the same time, this product uses genetically modified proteins A and G, which not only maintain their Ig affinity properties, but also remove the non-major binding domain of the natural protein itself to reduce non-specific binding. This product has a wide range of application, can be used in cell lysis fluid, cell secretory fluid supeatant, serum, animal ascites and other immune antigen samples.

Application

Immunoprecipitation (IP)
Purification of antibody

Features

Low background—little to no nonspecific binding, and no preclearing
Highly reproducible—uniform beads ensure the most consistent results
Highly sensitive—Magbeads technology is the most cited method for sensitive applications, such as ChIP and IP, of low abundance proteins
Fast and easy— no centrifugation or preclearing steps
Versatile—products for IP, Co-IP, pull-down, and ChIP assays.

Other Component

Components No.	Name	36417ES03	36417ES08
36417	rProtein A/G MagBeads (IP Grade)	1 mL	5 mL

Specification

Ligand	rProtein A/G
Binding Capacity	≥50 μg hIgG /mg
Particle size	1 μm
Concentration	10 mg/mL
Storage Buffer	PBS, 0.01% Tween-20, 0.02% NaN3

Smaller beads, Higher capacity

	Thermo Dynabeads™ Protein G/Protein A, 10003D/10001D	Yeasen Protein A/G MagBeads, 36417ES
Diameter	2.8 μm	1 μm
Concentration	30 mg/mL	10 mg/mL
Capacity	About 8 µg human IgG/mg beads	About 50µg human IgG /mg beads

Protocals And Manuals	Manual

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHD20800003-1	36417ES08	5mL	$395		Add to Cart
BHD20800003-2	36417ES03	1mL	$105		Add to Cart

Protein A/G MagBeads (IP Grade)

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