FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.The antibody reacts with FITC but not the carrier protein.
Clonality
polyclonal
Host
Rabbit
Immunogen
FITC conjugated to KLH
Immunogen Region
FITC conjugated to KLH
Isotype
IgG
Reactivity
Fitc
Recommended Dilution
0.5-1.0 ug/mL for elisa
Synonyms
Fluorescein Isothiocyanate
Form
liquid
Purification
Immunogen affinity purified
Purity
>=95% as determined by SDS-PAGE
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze / thaw cycles.)
Tested Application
0.5-1.0 ug/mL for elisa
Product Manual
<a href="https://www.fn-test.com/content/uploads/product/manuals/antibody/FNab10893.pdf" target="_blank">Data Sheet</ a>