• Sensitivity :
• 9.375pg/ml

• Detection Range :
• 15.625-1000pg/ml

• Reactivity :
• Human

• Assay Duration :
• 4 hours

• Assay Type :
• Sandwich

• Description :
• This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti MIP-3beta antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with MIP-3beta bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of MIP-3beta in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

• Alternative Names :
• C-C motif chemokine 19 ELISA Kit, Beta-chemokine exodus-3 ELISA Kit, CK beta-11 ELISA Kit, Epstein-Barr virus-induced molecule 1 ligand chemokine ELISA Kit, EBI1 ligand chemokine, ELC ELISA Kit, Macrophage inflammatory protein 3 beta ELISA Kit, MIP-3-beta ELISA Kit, Small-inducible cytokine A19 ELISA Kit, CCL19 ELISA Kit, ELC ELISA Kit, MIP3B ELISA Kit, SCYA19 ELISA Kit

• Detection Method :
• Double Antibody

• Kit Components :
• <table> <thead> <tr> <th>Kit Components</th> <th>Item</th> <th>Size(48T)</th> <th>Size(96T)</th> <th style="width: 40%;">Storage Condition for Opened Kit</th> </tr> </thead> <tbody> <tr> <td>E001</td> <td>ELISA Microplate(Dismountable)</td> <td>8x6</td> <td>8x12</td> <td>Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C</td> </tr> <tr> <td>E002</td> <td>Lyophilized Standard</td> <td>1vial</td> <td>2vial</td> <td>Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C</td> </tr> <tr> <td>E003</td> <td>Biotin-labeled Antibody(Concentrated, 100X)</td> <td>60ul</td> <td>120ul</td> <td rowspan="3">2-8°C (Avoid Direct Light)</td> </tr> <tr> <td>E034</td> <td>HRP-Streptavidin Conjugate(SABC, 100X) </td> <td>60ul</td> <td>120ul</td> </tr> <tr> <td>E024</td> <td>TMB Substrate</td> <td>5ml</td> <td>10ml</td> </tr> <tr> <td>E039</td> <td>Sample Dilution Buffer</td> <td>10ml</td> <td>20ml</td> <td rowspan="5">2-8°C</td> </tr> <tr> <td>E040</td> <td>Antibody Dilution Buffer</td> <td>5ml</td> <td>10ml</td> </tr> <tr> <td>E049</td> <td>SABC Dilution Buffer </td> <td>5ml</td> <td>10ml</td> </tr> <tr> <td>E026</td> <td>Stop Solution</td> <td>5ml</td> <td>10ml</td> </tr> <tr> <td>E038</td> <td>Wash Buffer(Concentrated, 25X) </td> <td>15ml</td> <td>30ml</td> </tr> <tr> <td>E006</td> <td>Plate Sealer</td> <td>3 pieces</td> <td>5 pieces</td> <td rowspan="2">&nbsp;</td> </tr> <tr> <td>E007</td> <td>Product Description</td> <td>1 copy</td> <td>1 copy</td> </tr> </tbody> </table>

• Recovery :
• Add a certain amount of MIP-3beta into the sample. Calculate the recovery by comparing the measured value with the expected amount of MIP-3beta in the sample.<br /><br /><table><thead> <tr> <th>Sample Type</th> <th>Recovery Range(%)</th> <th>Average(%)</th> </tr> </thead> <tbody> <tr> <td>serum(n=10)</td> <td>86-96</td> <td>91</td> </tr> <tr> <td>EDTA plasma(n=10)</td> <td>86-102</td> <td>92</td> </tr> <tr> <td>Heparin plasma(n=10)</td> <td>88-102</td> <td>95</td> </tr> </tbody></table>

• Linearity :
• Dilute the sample with a certain amount of MIP-3beta at 1:2, 1:4 and 1:8 to get the recovery range.<br /><br /><table><thead> <tr> <th>Sample Type</th> <th>1:2</th> <th>1:4</th> <th>1:8</th> </tr> </thead> <tbody> <tr> <td>serum(n=10)</td> <td>85-105%</td> <td>91-105%</td> <td>86-101%</td> </tr> <tr> <td>EDTA plasma(n=10)</td> <td>82-100%</td> <td>82-97%</td> <td>83-100%</td> </tr> <tr> <td>Heparin plasma(n=10)</td> <td>84-99%</td> <td>87-98%</td> <td>84-100%</td> </tr> </tbody></table>

• Specificity :
• Specifically binds with MIP-3beta , no obvious cross reaction with other analogues.

• Standard Curve :
• This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.) Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.<br /><br /><table> <thead> <tr> <th>STD.(pg/ml)</th> <th>OD-1</th> <th>OD-2</th> <th>Average</th> </tr> </thead> <tbody> <tr> <td>0</td> <td>0.096</td> <td>0.098</td> <td>0.097</td> </tr> <tr> <td>15.625</td> <td>0.211</td> <td>0.217</td> <td>0.214</td> </tr> <tr> <td>31.25</td> <td>0.273</td> <td>0.281</td> <td>0.277</td> </tr> <tr> <td>62.5</td> <td>0.421</td> <td>0.433</td> <td>0.427</td> </tr> <tr> <td>125</td> <td>0.606</td> <td>0.624</td> <td>0.615</td> </tr> <tr> <td>250</td> <td>0.97</td> <td>0.998</td> <td>0.984</td> </tr> <tr> <td>500</td> <td>1.459</td> <td>1.501</td> <td>1.48</td> </tr> <tr> <td>1000</td> <td>2.002</td> <td>2.06</td> <td>2.031</td> </tr> </tbody> </table>

• Components :
• Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

• Precision :
• Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.<br />Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.<br /><br /><table><thead> <tr> <th>Item</th> <th colspan="3">Intra-assay Precision</th> <th colspan="3">Inter-assay Precision </th> </tr> </thead> <tbody> <tr> <td>Sample</td> <td>1</td> <td>2</td> <td>3</td> <td>1</td> <td>2</td> <td>3</td> </tr> <tr> <td>n</td> <td>20</td> <td>20</td> <td>20</td> <td>20</td> <td>20</td> <td>20</td> </tr> <tr> <td>Mean (pg/ml)</td> <td>31.34</td> <td>129</td> <td>497</td> <td>31.87</td> <td>121.8</td> <td>507</td> </tr> <tr> <td>Standard deviation</td> <td>1.78</td> <td>6.82</td> <td>27.88</td> <td>1.65</td> <td>6.71</td> <td>26.11</td> </tr> <tr> <td>CV(%)</td> <td>5.68</td> <td>5.29</td> <td>5.61</td> <td>5.19</td> <td>5.51</td> <td>5.15</td> </tr> </tbody></table>

• Background :
• MIP-3β/ELC/CCL19/beta chemokine exodus-3/Beta-chemokine exodus-3/CC chemokine ligand 19/C-C motif chemokine 19/chemokine(C-C motif) ligand 19/CKb11/EBI1-ligand chemokine/ELCMIP-3-beta/Epstein-Barr virus-induced molecule 1 ligand chemokine/exodus-3/Macrophage inflammatory protein 3 beta/macrophage inflammatory protein 3-beta/MGC34433/MIP-3b/MIP3BCK beta-11/SCYA19EBI1 ligand chemokine/small inducible cytokine subfamily A(Cys-Cys), member 19/Small-inducible cytokine A19

• Stability :
• Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.<br /><br /><table><tbody> <tr> <td>ELISA kit(n=5)</td> <td>37°C for 1 month</td> <td>2-8°C for 6 months </td> </tr> <tr> <td>Average(%)</td> <td>80</td> <td>95-100</td> </tr> </tbody></table>

• Notes :
• For Research Use Only

• Usage Notes :
• <img alt="Assay Procedure Summary" height="265" src="https://s3.us-west-1.amazonaws.com/s3.biohippo.com/elisakits/fine-test/images/ff_Assay_Procedure_Summary.jpg" width="650" /> <ul class="czlc"><br /> <li> <span>Step 1:</span><br /> Take out the required plate wells, add 50ul Cap/Det Ab into each well, then add 50ul Standard or Sample into individual well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure thorough mixing then static incubate for 60 minutes at 37°C.<br /> </li><br /> <li> <span>Washing:</span><br /> Wash the plate twice without immersion.<br /> </li><br /> <li> <span>Step 2:</span><br /> Add 100ul HRP-Streptavidin (orange) into each well, seal the plate and static incubate for 30 minutes at 37°C.<br /> </li><br /> <li> <span>Washing: </span><br /> Wash the plate five times without immersion.<br /> </li><br /> <li> <span>Step 3:</span><br /> Add 90ul TMB substrate solution, seal the plate and static incubate for 10-20 minutes at 37°C. (Accurate TMB visualization control is required.)<br /> </li><br /> <li> <span>Step 4:</span><br /> Add 50ul stop solution. Read at 450nm immediately and calculate.<br /> </li><br /></ul>

• UniProt ID :
• Q99731

• Applications :
• ELISA

• Applications Notes :
• MIP-3beta ELISA Kit allows for the in vitro quantitative determination of MIP-3beta concentrations in serum, plasma, tissue homogenates and other biological fluids.

• Storage :
• 2-8°C(Sealed), Don't cryopreserve.