• Alternative Names :
• Tyrosine-protein kinase HCK (EC 2.7.10.2) (Hematopoietic cell kinase) (Hemopoietic cell kinase) (p59-HCK/p60-HCK) (p59Hck) (p61Hck), HCK

• Categories :
• Primary Antibodies

• Clonality :
• polyclonal

• Description :
• Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS.

• Host :
• Rabbit

• Immunogen :
• Recombinant Human Tyrosine-protein kinase HCK protein (1-220AA), Recombinant Human Tyrosine-protein kinase HCK protein (1-220AA)

• Involvement In Disease :
• Aberrant activation of HCK by HIV-1 protein Nef enhances HIV-1 replication and contributes to HIV-1 pathogenicity.; DISEASE: Note=Aberrant activation of HCK, e.g. by the BCR-ABL fusion protein, promotes cancer cell proliferation.

• Isotype :
• IgG

• Raised In :
• Rabbit

• Reactivity :
• Human

• Regulatory :
• RUO

• Relevance :
• Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS.

• Species :
• Homo Sapiens (Human)

• Subcellular Location :
• Isoform 1: Lysosome, Membrane, Lipid-anchor, Cell projection, podosome membrane, Lipid-anchor, Cytoplasm, cytosol, Note=Associated with specialized secretory lysosomes called azurophil granules, At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated, SUBCELLULAR LOCATION: Isoform 2: Cell membrane, Lipid-anchor, Membrane, caveola, Lipid-anchor, Cell junction, focal adhesion, Cytoplasm, cytoskeleton, Golgi apparatus, Cytoplasmic vesicle, Lysosome, Nucleus, Isoform 1: Lysosome, Membrane, Lipid-anchor, Cell projection, podosome membrane, Lipid-anchor, Cytoplasm, cytosol, Note=Associated with specialized secretory lysosomes called azurophil granules, At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated, SUBCELLULAR LOCATION: Isoform 2: Cell membrane, Lipid-anchor, Membrane, caveola, Lipid-anchor, Cell junction, focal adhesion, Cytoplasm, cytoskeleton, Golgi apparatus, Cytoplasmic vesicle, Lysosome, Nucleus

• Uniprot :
• P08631

• Database Links :
• 
  

  HGNC:4840

  OMIM:142370

  KEGG:hsa:3055

  STRING:9606.ENSP00000365012

  UniGene:Hs.655210

  ,
  

  HGNC:4840

  OMIM:142370

  KEGG:hsa:3055

  STRING:9606.ENSP00000365012

  UniGene:Hs.655210

• Function :
• Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS., Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS.

• Pathway :
• Chemokine signaling pathway Fc gamma R-mediated phagocytosis

• Protein Families :
• Protein kinase superfamily, Tyr protein kinase family, SRC subfamily, Protein kinase superfamily, Tyr protein kinase family, SRC subfamily

• Research Area :
• Signal Transduction, Signal Transduction

• Tissue Specificity :
• Detected in monocytes and neutrophils (at protein level). Expressed predominantly in cells of the myeloid and B-lymphoid lineages. Highly expressed in granulocytes. Detected in tonsil.

• Buffer :
• PBS with 0.02% sodium azide, 50% glycerol, pH7.3., PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Form :
• Liquid

• Format :
• Liquid, Liquid

• Purification :
• Antigen Affinity Purified, Antigen Affinity Purified

• Purity :
• Antigen Affinity Purified

• Storage :
• Upon receipt, store at -20°C or -80°C. Avoid repeated freeze., Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

• Storage Buffer :
• 
  
  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Datasheet :
• 

• ELISA Protocol :

• Immunohistochemistry (IHC) Protocol :