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RFP-Expressing Mouse Primary Lymphatic Endothelial Cells

SKU : BHC16402999

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Cell Biologics, Inc.

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Mfr.No.	C57-6092RFP
Description	RFP-Expressing Mouse Primary Lymphatic Endothelial Cells from Cell Biologics are isolated from the lymph nodes of pathogen-free laboratory C57BL/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10^6 cells per ml and is delivered frozen. RFP-Expressing Mouse Primary Lymphatic Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R&D System) or CD31/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.
Cell Type	Endothelial Cells
Cell Models	RFP
Media	Complete Endothelial Cell Medium w/ Kit 500 ml
Tissue	Lymphatic
Notes	Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Species	Mouse

Applications	Immunologic Research

Product Use	RFP-Expressing Mouse Primary Lymphatic Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Product Manual	Cell Culture Protocol MSDS

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SKU	Supplier No.	Size	Your price	Quantity
BHC16402999	C57-6092RFP	0.5x106 Cells	$890		Add to Cart

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