Rat Pulmonary Vein Smooth Muscle Cells from AcceGen are isolated from tissue of Sprague-Dawley Rats. Rat Pulmonary Vein Smooth Muscle Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in AcceGen's Cell Culture Medium for 3-7 days. Cultures are then eXpanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in VIals. Each VIal contains at least 1X10^6 cells per ml and is delIVered frozen.Cells are negatIVe for bacteria, yeast, fungi, and mycoplasma and are characterized by immunofluorescent staining with antibodies to a-smooth muscle actin. Cells can be eXpanded on a multiwell culture plate ready for eXperiments under the cell culture conditions specified by AcceGen.Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with smooth muscle cell cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derIVatIVes for desired research applications.
Categories
Primary Cells
Species
Rat
Applications
For research use only
Shipping
Dry Ice
Storage
Liquid Nitrogen
Quality Control
All cells test negatIVe for mycoplasma, bacteria, yeast, and fungi.