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Rat Primary Pulmonary Artery Smooth Muscle Cells

SKU : BHC16401207

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Cell Biologics, Inc.

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Mfr.No.	RA-6083
Description	Rat Primary Pulmonary Artery Smooth Muscle Cells from Cell Biologics are isolated from the Pulmonary Artery tissues of 6-8 weeks old laboratory SpragueDawley rat and grown in gelatin pre-coated tissues culture flask with Cell Biologics Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 1x10^6 cells per ml and is delivered frozen. Rat Primary Pulmonary Artery Smooth Muscle Cells are characterized by immunofluorescence staining with antibody of ?-smooth muscle actin (A2547, Sigma) and are negative for bacteria, yeast, fungi, and mycoplasma. These cells can be expanded for 2-3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.
Cell Origin	Rat
Cell Type	Smooth Muscle Cells
Application	Rat Primary Pulmonary Artery Smooth Muscle Cells can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Disclaimer	Investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Frozen	1×10⁶cells
General Information	Rat Primary Pulmonary Artery Smooth Muscle Cells are characterized by immunofluorescent method with antibody to ?-smooth muscle actin. Rat Primary Pulmonary Artery Smooth Muscle Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Cell Biologics (Cells may be expanded for 2 or 3 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics). Repeated freezing and thawing of cells is not recommended.
Media	Complete Smooth Muscle Cell Medium w/ Kit 500 ml
Tissue	Pulmonary Artery
Notes	Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Species	Rat

Applications	Pulmonary Research

Product Use	Rat Primary Pulmonary Artery Smooth Muscle Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Product Manual	Cell Culture Protocol MSDS

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SKU	Supplier No.	Size	Your price	Quantity
BHC16401207	RA-6083	0.5x106 Cells	$410		Add to Cart

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