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Home / Cells / Primary Cells

Rat Hepatic Stellate Cells

SKU : BHC16404246

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Cell Biologics, Inc.

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Mfr.No.	RA-6242
Description	Rat Primary Hepatic Stellate Cells from Cell Biologics are isolated from Sprague-Dawley rat liver tissues and grown in tissue culture plates with Cell Biologics Cell Culture Medium. Cells at passage 0 are cryo-preserved and each vial contains 0.2x10^6 cells. Vitamin A droplets in Hepatic Stellate Cells are visualized by blue autofluorescence when excited with UV light. These cells can be used for experiments under the cell culture conditions specified by Cell Biologics. Hepatic Stellate Cells are not recommended for long-term cultures since these cells would differentiate into fibroblat-like cells after plating, and cells will not proliferate in culture and can not be passaged.
Cell Type	Hepatic Stellate Cells
Freeze Thaw Recovery	Quickly thaw cells in cryo-vial by incubating them in a 37°C water bath for <1 min until there is just a small bit of ice left in the vial.Promptly remove the vial and wipe it down with 70% ethanol.Transfer cells from the vial to a sterile centrifuge tube. Add 5 ml of pre-warmed Cell Biologics Cell Culture Medium. Flush the vial with an additional 0.5-1 ml of medium to ensure complete transfer of cells to the centrifuge tube.Centrifuge cells at 200 g for 5 minutes.Aspirate the supernatant and resuspend the cell pellet in 3 ml of Cell Biologics Cell Culture Growth medium.Add resuspended cells into a tissue-culture treated plate (to seed on 2-3 wells of a 12-well plate or 1 well of 6-well plate).Place a plate in a humidified, 5%-CO2 incubator at 37°C until experiments.Change fresh cell culture medium every 24-48 hours.Cells should be checked daily under a microscopy to verify appropriate cell morphology.Note
Media	Complete Hepatic Stellate Cell Medium /w Kit (500 ml)
Tissue	Liver
Notes	Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Species	Rat

Applications	Hepatologic Research

Product Use	Rat Primary Hepatic Stellate Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Product Manual	Cell Culture Protocol MSDS

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SKU	Supplier No.	Size	Your price	Quantity
BHC16404246	RA-6242	0.2x106 Cells	$560		Add to Cart

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