Human umbilical vein endothelial cells (HUVECs) serve as a model system for the study of endothelial cell function and disease and are commonly used in studies involving angiogenesis; homeostasis; inflammatory disorders; atherosclerosis; cancer and cardiovascular-related diseases.
HUVECs were isolated from the umbilical cord vein of a single donor (female offspring). Cells are passaged for purity then cryopreserved and delivered frozen. Each vial contains 1 x 106 cells in 1 mL volume. Cells are characterized by flow cytometry for CD31/CD144/CD146. HUVECs are negative for mycoplasma/yeast/fungi/bacteria. HIV-1 as well as HIV-2 and HBV are not detected. Cells are assured to further culture using the protocol provided.
Human Material PrecautionTissues used for cell isolation were acquired with informed consent and adhere to HIPAA standards for human privacy protection. All human biological material (including human cells) should always be handled as potentially infectious using at least Biosafety Level 2 precautions; we recommend that appropriate safety procedures be followed using the same biosafety protocol used with known infectious material. Viral testing cannot prove the absence of a latent viral genome.
Gender
Female
Growth Properties
Adherent
Quantity
1x106 cells / 1.0 ml
Morphology
Polygonal
Organism
Homo sapiens
Species
Human
Propagation
Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM5482) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO?.
Source Organ
Reproductive
Shipping
Dry Ice
Storage
-180°C
Reviews of Human Primary Umbilical Vein Endothelial Cells