Generally mature adipocytes isolated from Human tissue cannot survive 24 hours after isolation. In order to facilitate research in cell energy storage and metabolic homeostasis, abm provides mature adipocytes differentiated from pre-adipocytes. These mature adipocytes are shipped as live cells in a T25 flask 7-10 days after induction of differentiation and small oil droplets will be visible and will continue to accumulate into big locules. Upon cell arrival, it is recommended to use the adipocytes within 3 weeks.
Cell Type
Primary Cells
Disease
Normal
Gender
Male
Growth Properties
Adherent
Quantity
5x105 cells / 1.0 ml
Tissue
Adipose
Morphology
Polygonal
Species
Human (H. sapiens)
Propagation
The base medium for this cell line is Prigrow X series medium available at abm, Cat. No. TM4000. Carbon dioxide (CO2): 5%, Temperature: 37.0
Applications
For Research Use Only
Subculture Protocol
Human Normal Subcutaneous Adipocytes can be split into different culture vessels (i.e. multi-well plates) for experimental needs, but differentiated adipocytes will not proliferate in vitro.
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