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Human Primary Skeletal Muscle Cells

SKU : BHC10900163

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Applied Biological Materials (abm) Inc.

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Mfr.No.	T4069
Cell Type	Primary Cells
Biosafety Level	2
Disclaimer	1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 2. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 ?g, Cat.# C207, $450.00) or cell lysate (100 ?g, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 3. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 4. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 5. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 6. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Disease	Normal
Donor History	Normal tissue
Gender	Male
Growth Properties	Adherent, multipolar
Growth Conditions	PriCoat?T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow X Series Medium (TM4069) + 1% Penicillin/Streptomycin Solution (G255), 37.0?, 5% CO?.Note:?ells have a finite lifespan?n vitro and can be propagated for up to 10 population doublings.
Quantity	5x105 cells / 1.0 ml
Tissue	Skeletal Muscle
Morphology	Multipolar
Notes	1. Visually examine the packaging containers for signs of leakage or breakage. 2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130?, preferably in liquid nitrogen vapor phase storage, until ready for use.To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130? or in liquid nitrogen vapor phase. Do not store at -70?, as it will result in loss of viability.
Organism	Human (H. sapiens)
Species	Human (H. sapiens)
Propagation	Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is Prigrow X series medium available at abm, Cat. No. TM4068. Carbon dioxide (CO2): 5%, Temperature: 37.0
Cryopreservation	Cryopreservation Medium (TM024), or complete growth media with 10% DMSO.

Applications Range	Research Use Only.
Applications	For Research Use Only

Shipping	Ship with dry ice.
Product Format	Frozen
Product Use	This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Storage	Vapor phase of liquid nitrogen, or below -130?.

Thawing Protocol	1. Prepare desired culture vessel(s) as per recommended seeding density by adding the appropriate volume of complete growth media. Allow vessel(s) to equilibate to the recommened conditions (37.0?, 5% CO?)?or at least 30 minutes. 2. Thaw cells quickly in a 37? water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination 3. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions. 4. Transfer the cell suspension into the culture vessels prepared in Step 1. Ensure cells are evenly distributed within the culture flask.?br />5. Incubate the cells at the recommended conditions. 6. Do not centrifuge cells to remove DMSO as this action will damage cells.?br />7. Change the media with fresh complete growth media after 24 hours.
Subculture Protocol	Note: cells have a finite lifespan in vitro and can be propagated for up to 10 population doublings.?olumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 50-70% confluent and contains many mitotic figures.?. Pre-warm all reagents to room temperature. Prepare culture vessel(s) by adding the required amount of complete growth media and allowing vessel(s) to equilbrate to the recommended?onditions (37.0?, 5% CO?)?or at least 30 minutes. 2. Aspirate the culture media, and rinse the cells with 5-10 ml of HEPES-BSS.?br>3. Add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel. 4. Observe the cells under a microscope to confirm detachment (typically within 2-6 minutes). Cells that are difficult to detach can be incubated at 37?, for several minutes to facilitate detachment. 5. Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel. 6. Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 220xg for 5 minutes.?br>7. Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Perform a cell count and add appropriate aliquots of the cell suspension to the prepared culture vessels, as per the recommended seeding density. 8. Incubate the cells at the recommended conditions. 9. Change growth media every other day, thereafter.

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SKU	Supplier No.	Size	Your price	Quantity
BHC10900163	T4069	5x10^5 cells / 1.0 ml	-		Add to Quote

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