Human Primary Pancreatic islets are provided fresh upon order and shipped as live cells. The Pancreatic islets consist a cluster of various hormone-producing cells, including 40%-60% pancreatic beta cells. The percentage of the beta cells varies between lot-to-lot preparations and can be determined as an added service. Upon cell arrival, it is recommended to use the cells within 7 days. Human primary Pancreatic islets is an ideal model for long-term islet grafting survival studies and can provide insight into development of novel therapies for other pancreas related diseases. Please contact our order desk for a quotation based on the IEQ required for your experiment. An IEQ or the islet equivalent is a standardized way to describe the islet size of 100 micron. In a case where a donor yields smaller islets, the customer may receive more actual islets than the ordered IEQ. The minimum IEQ per order is 1,000.
Cell Type
Primary Cells
Disease
Normal
Gender
Donor Info Not Disclosed
Growth Properties
Suspension
Quantity
Per IEQ
Tissue
Digestive
Morphology
Suspension
Species
Human (H. sapiens)
Propagation
The media supplied during shipment is not sufficient for the cells and the customer will need to buy Cat. No. TM0199-A for IMMEDIATE Islet Recovery upon delivery and TM0199-B for culturing following recovery- Cat. No. TM0199-A for Islet Recovery as it enables the islets to round up with increased glucose responsiveness within the first 48 hours of recovery.- Cat. No. TM0199-B for Long Term Culturing as it is enhanced to reduce islet chaining and minimize islet fusion.- Cat. No. TM0199-C for Islet Cold Culture Media for transportation only. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
Applications
For Research Use Only
Subculture Protocol
Human Primary Pancreatic Islets can be split into different culture vessels (i.e. multi-well plates) for experimental needs, but they will not proliferate in vitro . Experiments should be conducted within 7 days upon cell arrival.
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