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Human Primary Hepatocytes

SKU : BHC10900120

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Applied Biological Materials (abm) Inc.

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Mfr.No.	T0051
Cell Type	Primary Cells
Biosafety Level	2
Disclaimer	1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]. 2. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 ?g, Cat.# C207, $450.00) or cell lysate (100 ?g, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 3. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 4. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 5. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. 6. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
Disease	Idiopathic Pulmonary Fibrosis
Donor History	Normal tissue
Growth Properties	Adherent, round
Growth Conditions	PriCoat?ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. PriGrow X Series Medium for T0051 (TM0051)?+ 1% Penicillin/Streptomycin Solution (G255), 37.0?, 5% CO?.Note: Cells cannot be subcultured or passaged; confluent monolayer can be maintained for up to 7 days.
Quantity	5 x 105 / 1.0 ml
Tissue	Liver
Morphology	Epithelial
Notes	1. Visually examine the packaging containers for signs of leakage or breakage. 2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130?, preferably in liquid nitrogen vapor phase storage, until ready for use.To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130? or in liquid nitrogen vapor phase. Do not store at -70?, as it will result in loss of viability.
Organism	Human (H. sapiens)
Species	Human (H. sapiens)
Propagation	Use of PriCoat T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is Prigrow X medium at abm (TM5472). Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
Cryopreservation	Cryopreservation Medium (TM024), or complete growth media with 10% DMSO.

Applications Range	Research Use Only.
Applications	For Research Use Only

Shipping	Ship with dry ice.
Product Format	Frozen
Product Use	This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Storage	Vapor phase of liquid nitrogen, or below -130?.

Thawing Protocol	1. Thaw cells quickly in a 37? water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination. 2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions. 3. Transfer the cell suspension into a 15ml sterile conical tube containing 10ml of pre-warmed, TM0051-T (Hepatocyte Thawing Medium). Centrifuge cells at 100xg for 5 minutes. 4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in 5 ml of TM0051-P (Hepatocyte Plating Medium) and seed as desired into well plates (96 wells to 6 wells). 5. Incubate the cells at the recommended conditions and allow cells to attach for an additional 3-4 hours, undistrubed. Gently aspirate non-attached cells and add fresh TM0051-P. Allow cells to culture undisturbed for an additional 12-16 hours.?br />6. Replace media with prewarmed?M0051-M (Hepatocyte Maintenance Medium) daily, thereafter.
Subculture Protocol	Cells cannot be subcultured or passaged.

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SKU	Supplier No.	Size	Your price	Quantity
BHC10900120	T0051	5x10^5 cells / 1.0 ml	-		Add to Quote

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