Primary human immature dendritic cells (DCs) were derIVed from immunomagnetically selected peripheral blood (PB) monocytes. Monocytes were cultured in RPMI 1640 Medium with 10% fetal boVIne serum (FBS), GM-CSF and IL-4 for 5 days to generate immature DCs. PB was collected using one of the following anticoagulants: acid-citrate-deXtrose (ACD), acid-citrate-deXtrose solution A (ACDA), citrate-phosphate-deXtrose (CPD), citrate-phosphate-double-deXtrose (CP2D), or citrate-phosphate-deXtrose-adenine (CPDA).
Categories
Primary Cells
Species
Human
Applications
For research use only
Shipping
Dry Ice
Storage
Liquid Nitrogen
Quality Control
All cells test negatIVe for mycoplasma, bacteria, yeast, and fungi.
Reviews of Human Peripheral Blood Immature Dendritic Cells