A specialized somatic stem cell (SSC) population in the human endometrium, often identified by using the Side Population (SP) technique, is thought to mediate the endometrial regeneration. The Human Endometrial Somatic Stem Cells are established from human endometrial side population cells, specifically from the Epithelial fraction (ICEp; Cat. No. T4107) or the Stromal fraction (ICEs; Cat. No. T4108). Both cell types retain the ability to undergo osteogenic and adipogenic differentiation in vitro . After adipogenic induction, the cells show lipid accumulation in the cytoplasm and increased expression of lipoprotein lipase (LPL). When under osteogenic induction, the cells stain positive for bonesialoprotein (BSP) and show increased expression of osterix. Furthermore, both cell types are capable of reconstructing total endometrial-like tissue in vivo when transplanted beneath the renal capsule of NOD-SCID mice. The Human Endometrial Somatic Stem Cells serve as a reliable model to test relevant targets for endometrial physiology and pathology.
Cell Type
Primary Cells
Disclaimer
1. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final.2. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a replacement is possible at a cost (plus shipping).3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.
Disease
Normal
Gender
Donor Info Not Disclosed
Growth Properties
Adherent
Quantity
5x105 cells / 1.0 ml
Tissue
Reproductive
Morphology
Fibroblast-like
Species
Human (H. sapiens)
Propagation
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is Prigrow IV medium available in ABM, Cat. No. TM004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, L-glutamine to 2mM and Penicillin/Streptomycin(G255). Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. * abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Applications
For Research Use Only
Reviews of Human Endometrial Somatic Stem Cells (Epithelial Fraction hESP-ICEp)