Human Primary Bladder Epithelial Cells from AcceGen are isolated from normal human bladder tissue. Human Primary Bladder Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in AcceGen’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then eXpanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in VIals. Each VIal contains at least 0.5×10^6 cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody.
Categories
Primary Cells
Species
Human
Applications
For research use only
Shipping
Dry Ice
Storage
Liquid Nitrogen
Quality Control
All cells test negatIVe for mycoplasma, bacteria, yeast, and fungi.