Mouse Heart Microvascular Endothelial cells (mHtMECs) are isolated from hearts of 3-week old male C57BL/6 mice and GFP eXpressing mHtMECs-GFP were selected from puromycin resistant cells after transfected with lentIVIral eXpressing GFP. Cells are shipped in a frozen VIal (proVIded @ passage 3, with ; 5 X 105 cells/VIal). mHtMECs-GFP are cultured with specially formulated Endothelial Growth Medium. Since the cells have limited proliferation capacity (cells may be eXpanded for a maXimum of 2-3 passages) in VItro, long term culture of mHtMECs is not recommended, even cultured following the detailed protocol described below.
Categories
Primary Cells
Species
Mouse
Applications
For research use only
Shipping
Dry Ice
Storage
Liquid Nitrogen
Quality Control
All cells test negatIVe for mycoplasma, bacteria, yeast, and fungi.
Reviews of GFP-Mouse Heart Microvascular Endothelial Cells