HRMPCs are initiated by elutriation from dissociated normal human retinal tissue and transfected with GFP-LentIVIral particles at passage 2. Puromycin resistant GPF-HRMPCs were selected and shipped in frozen VIals (the cells are proVIded @ passage 4-5). Pericytes growth medium is recommended for cell culture and these cells have an average population doubling capacity ; 6 when cultured following the detailed protocol described below.
Categories
Primary Cells
Species
Human
Applications
For research use only
Shipping
Dry Ice
Storage
Liquid Nitrogen
Quality Control
All cells test negatIVe for mycoplasma, bacteria, yeast, and fungi.
Reviews of GFP-Human Retinal Microvascular Pericytes