HLiStCs are initiated by hepatic artery perfusion digestion with collagenase. HLiStCs are separated from the miXture of hepatic sinusoidal MV endothelial cells and Kupffer cells in the digestion solution. GFP-HLiStCs are selected from Puromycin resistant HLiStCs after infected with GFP eXpressing lentIVIral particles. The cells are shipped in frozen VIals (the cells are proVIded @ passage 3). Stellate-Growth medium is recommended for cell culture and these cells have a minimum average population doubling capacity ; 8 when cultured following the detailed protocol described below.
Categories
Primary Cells
Species
Human
Applications
For research use only
Shipping
Dry Ice
Storage
Liquid Nitrogen
Quality Control
All cells test negatIVe for mycoplasma, bacteria, yeast, and fungi.