Mesenchymal stem cells (MSCs) are multipotent stem cells with a high capacity for self-renewal. They can develop into specialized cells of the body such as chondrocytes/osteoblasts/adipocytes and their easy accessibility as well as exceptional genomic stability and capacity for tissue repair with few immunological and ethical issues make them a favorable cell type for experimental and clinical use. Umbilical cord-derived mesenchymal stem cells (UC-MSCs) have advantages over traditional sources of MSCs including ease of collection; faster self-renewal; longer multipotency and additional immunosuppressive properties; making them potentially beneficial in regenerative medicine and tissue engineering. UC-MSCs were isolated from the umbilical cord tissue of a neonatal (day 0) male dog. The cells are passaged for purity then cryopreserved and delivered frozen. Each vial contains 1 x 106 cells in 1 mL volume. Cells are characterized by flow cytometry for CD90/CD105/CD44/CD45/CD34. UC-MSCs are negative for mycoplasma/yeast/fungi/bacteria. Cells are assured to further culture using the protocol provided.
Cell Type
Primary Cells
Gender
Male
Growth Properties
Adherent
Quantity
1x106 cells / 1.0 ml
Tissue
Reproductive
Morphology
Polygonal
Species
Dog (Canine)
Propagation
Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The medium for this cell line is Prigrow XIV Medium at abm; Cat. No. TM014. Change media every 2-3 days.Carbon dioxide (CO2): 5%; Temperature: 37.0C.
Applications
For Research Use Only
Reviews of Dog Primary Umbilical Cord-derived Mesenchymal Stem Cells