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Home / Cells / Primary Cells

Diabetic Mouse Small Intestinal Epithelial Cells

SKU : BHC16400902

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Cell Biologics, Inc.

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Mfr.No.	MD-6051
Description	Diabetic MouseSmall Intestinal Epithelial Cells fromCell Biologics are isolated from the small intestinal tissues of Diabetic (db/db) mice.Diabetic MouseSmall Intestinal Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated inCell Biologics Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10⁶cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.
Cell Origin	Mouse
Cell Type	Epithelial Cells
Application	Mouse Primary Diabetic Small Intestinal Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Frozen	0.5×10⁶cells
General Information	Mouse Primary Diabetic Small Intestinal Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD), ZO-1 (Catalog 617300, Life Technology). Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Tissue	Small Intestinal

Storage	Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.

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SKU	Supplier No.	Size	Your price	Quantity
BHC16400902	MD-6051	Frozen Vial	$610		Add to Cart

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