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Home / Cells / Primary Cells

Diabetic Mouse Kidney Fibroblasts

SKU : BHC16400913

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Cell Biologics, Inc.

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Mfr.No.	MD-6016
Description	Diabetic MouseKidney Fibroblasts fromCell Biologics are isolated from the kidney of Diabetic (db/db) mice(8 weeks).Diabetic MouseKidney Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated inCell Biologics Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10⁶ cells per ml.
Cell Origin	Mouse
Cell Type	Fibroblasts
Application	Diabetic Mouse Kidney Fibroblasts can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Frozen	0.5×10⁶cells
General Information	Diabetic Mouse Kidney Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 (Millipore USA) by immunofluorescence staining. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics . Repeated freezing and thawing of cells is not recommended.
Tissue	Kidney

Storage	Cell Biologics Products Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Primary cells should never be kept at -20 °C.

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SKU	Supplier No.	Size	Your price	Quantity
BHC16400913	MD-6016	Frozen Vial	$590		Add to Cart

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