Cynomolgus Monkey Primary Bladder Microvascular Endothelial Cells from Cell Biologics are isolated from Cynomolgus monkey bladder tissue. Cynomolgus Monkey Primary Bladder Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in Cell Biologics Culture Complete Growth Medium generally for 3-7 days. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, followed by the application of magnetic beads pre-coated with secondary antibody.
Cell Origin
Cynomolgus Monkey
Cell Type
Endothelial Cells
Application
Cynomolgus Monkey Primary Bladder Microvascular Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Disclaimer
Appropriate safety procedures should always be used with this material. Although Cynomolgus Monkey Primary Bladder Microvascular Endothelial Cells are isolated from normal monkey tissue, investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.
Frozen
0.5×106cells
General Information
Cynomolgus Monkey Primary Bladder Endothelial Cells from Cell Biologics display typical cobblestone with large dark nuclei appearance under light microscopy. Cells are tested for LDL uptake positive by immunofluorescence staining. Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded on a plate ready for experiments under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Tissue
Bladder
Product Use
Cynomolgus Monkey Primary Bladder Microvascular Endothelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage
Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request. Never can primary cells be kept at -20 °C.
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