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Home / Cells / Primary Cells

C57BL/6 Mouse Spleen Fibroblasts

SKU : BHC18000312

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AcceGen Biotech

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Mfr.No.	ABC-TC3245
Description	C57BL/6 Mouse Primary Spleen Fibroblasts from AcceGen are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Spleen Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen Culture Complete Growth Medium generally for 3-7 days. Cultures are then eXpanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in VIals. Each VIal contains at least 1X10^6 cells per ml and is delIVered frozen.Mouse Primary Spleen Fibroblasts are negatIVe for bacteria, yeast, fungi, and mycoplasma. Cells are tested for eXpression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be eXpanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derIVatIVes for desired research applications.
Categories	Primary Cells
Species	C57BL/6 Mouse
Applications	For research use only

Shipping	Dry Ice
Storage	Liquid Nitrogen
Quality Control	All cells test negatIVe for mycoplasma, bacteria, yeast, and fungi.

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SKU	Supplier No.	Your price	Quantity
BHC18000312	ABC-TC3245	-		Add to Quote

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