Mouse Pulmonary Vein Vascular Smooth Muscle Cells from AcceGen are isolated from tissue of pathogen-free laboratory mice. Mouse Pulmonary Vein Vascular Smooth Muscle Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen's Cell Culture Medium generally for 3-7 days. Cultures are then eXpanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in VIals. Each VIal contains at least 1X10^6 cells per ml and is delIVered frozen.Mouse Pulmonary Vein Vascular Smooth Muscle Cells are characterized by immunofluorescent cell staining with antibodies of a-smooth muscle actin and are negatIVe for bacteria, yeast, fungi, and mycoplasma.Cells can be eXpanded on a multiwell culture plate ready for eXperiments under the cell culture conditions specified by AcceGen.Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derIVatIVes for desired research applications.
Categories
Primary Cells
Species
C57BL/6 Mouse
Applications
For research use only
Shipping
Dry Ice
Storage
Liquid Nitrogen
Quality Control
All cells test negatIVe for mycoplasma, bacteria, yeast, and fungi.
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