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C57BL/6 Mouse Primary Cholangiocytes

SKU : BHC16400428

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Cell Biologics, Inc.

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Mfr.No.	C57-6044C
Description	C57BL/6 Mouse Primary Cholangiocytes fromCell Biologics are isolated from the bile duct tissue of pathogen-free laboratory mice. C57BL/6 Mouse Primary Cholangiocytes are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated inCell Biologics Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10⁶ cells per ml and is delivered frozen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.
Cell Origin	Mouse
Cell Type	Cholangiocytes
Application	C57BL/6 Mouse Primary Cholangiocytes can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Disclaimer	Although C57BL/6 Mouse Primary Cholangiocytes are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Frozen	0.5×10⁶cells
General Information	C57BL/6 Mouse Primary Cholangiocytes are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD) or ZO-1 (Catalog 617300, Life Technology). C57BL/6 Mouse Primary Cholangiocytes are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics . Repeated freezing and thawing of cells is not recommended.
Tissue	Cholangiocytes

Product Use	C57BL/6 Mouse Primary Cholangiocytes from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures or for therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.Never can primary cells be kept at -20 °C.

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SKU	Supplier No.	Size	Your price	Quantity
BHC16400428	C57-6044C	Frozen Vial	$560		Add to Cart

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