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Home / Cells / Primary Cells

C57BL/6 Mouse Embryonic Vein Fibroblasts

SKU : BHC16400980

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Cell Biologics, Inc.

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Mfr.No.	C57-6069E
Description	C57BL/6 Mouse Embryonic Vein Fibroblasts fromCell Biologics are isolated from vein tissue of pathogen-free laboratory miceat embryonic day 14.5-19. Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated inCell Biologics Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded.Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10⁶cells per ml and is delivered frozen.
Cell Origin	Mouse
Cell Type	Fibroblasts
Application	C57BL/6 Mouse Embryonic Vein Fibroblasts can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Disclaimer	Investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Frozen	0.5×10⁶cells
General Information	C57BL/6 Mouse Embryonic Vein Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 (Millipore USA) by immunofluorescence staining. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Tissue	Vein

Product Use	C57BL/6 Mouse Embryonic Vein Fibroblasts from Cell Biologics are distributed for internal research purposes only. Our products are not authorized for human use, for in vitro diagnostic procedures, or for therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics, without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request. Primary cells can never be kept at -20 °C.

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SKU	Supplier No.	Size	Your price	Quantity
BHC16400980	C57-6069E	Frozen Vial	$490		Add to Cart

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