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Home / Cells / Primary Cells

BKS DB Mouse Spleen Endothelial Cells

SKU : BHC18001351

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AcceGen Biotech

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Mfr.No.	ABC-TC5496
Description	BKS db Mouse Spleen Endothelial Cells are isolated from the spleen of Mice homozygous for the diabetes spontaneous mutation (Lepr/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoRIemic. BKS db Mouse Spleen Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in AcceGen's Culture Complete Growth Medium generally for 3-7 days. Cultures are then eXpanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in VIals. Each VIal contains at least 0.5×106 cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody.
Categories	Primary Cells
Species	BKS DB Mouse
Applications	For research use only

Shipping	Dry Ice
Storage	Liquid Nitrogen
Quality Control	All cells test negatIVe for mycoplasma, bacteria, yeast, and fungi.

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