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BALB/c Mouse Primary Colonic Epithelial Cells

SKU : BHC16400453

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Cell Biologics, Inc.

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Mfr.No.	BALB-5047
Description	BALB/c Mouse Primary Colonic Epithelial Cells from Cell Biologics are isolated from the colonic tissues of pathogen-free laboratory mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10^6 cells per ml and is delivered frozen. BALB/c Mouse Primary Colonic Epithelial Cells are characterized by immunofluorescent staining with E-cadherin (Catalog 610182, BD) or ZO-1 (Catalog 617300, Life Technology) antibody. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.
Cell Origin	Mouse
Cell Type	Epithelial Cells
Application	BALB/c Mouse Primary Colonic Epithelial Cells can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Disclaimer	Although BALB/c Mouse Primary Colonic Epithelial Cells are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from Cell Biologics with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Frozen	0.5×10⁶cells
General Information	BALB/c Mouse Primary Colonic Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin (Catalog 610182, BD) or ZO-1 (Catalog 617300, Life Technology). BALB/c Mouse Primary Colonic Epithelial Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics . Repeated freezing and thawing of cells is not recommended.
Media	Complete Epithelial Cell Medium w/ Kit 500 ml
Tissue	Colonic
Notes	Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.
Species	Mouse
Species details	BALB/c Mouse

Applications	Digestive Research

Product Use	BALB/c Mouse Primary Colonic Epithelial Cells from Cell Biologics are distributed for research purposes only. Our products are not authorized for human use, for in vitro diagnostic or therapeutic procedures. Transfer or resale of any Cell Biologics cells or products from the purchaser to other markets, organizations or individuals is prohibited by Cell Biologics without the companys written consent. Cell Biologics Terms and Conditions must be accepted before submitting an order.
Storage	Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Product Manual	Cell Culture Protocol MSDS

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SKU	Supplier No.	Size	Your price	Quantity
BHC16400453	BALB-5047	1x106 Cells	$560		Add to Cart

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