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B129 Mouse Primary Coronary Artery Fibroblasts

SKU : BHC16400599

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Cell Biologics, Inc.

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Mfr.No.	B129-7049CO
Description	B129 Mouse Primary Coronary Artery Fibroblasts fromCell Biologics are isolated from coronary artery tissue of pathogen-free laboratory B129S2/SvPasCrl mice. B129 Mouse Primary Coronary Artery Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated inCell Biologics Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded.Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10⁶cells per ml and is delivered frozen.
Cell Origin	Mouse
Cell Type	Fibroblasts
Application	B129 Mouse Primary Coronary Artery Fibroblasts can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Weste blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
Frozen	1×10⁶cells
General Information	B129 Mouse Primary Coronary Artery Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 (Millipore USA) by immunofluorescence staining. B129 Mouse Primary Coronary Artery Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.
Tissue	Coronary Artery

Storage	Cell Biologics Products Cell Biologics ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request. Never can primary cells be kept at -20 °C.

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SKU	Supplier No.	Size	Your price	Quantity
BHC16400599	B129-7049CO	Frozen Vial	$510		Add to Cart

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