Aged Rat Primary Aortic Endothelial Cells from AcceGen are isolated from aorta of 74 week old laboratory Sprague-Dawley rat. Aged Rat Primary Aortic Endothelial Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in AcceGen's Culture Complete Growth Medium generally for 3-7 days. Cultures are then eXpanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in VIals. Each VIal contains at least 1X106 cells per ml and is delIVered frozen. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 (CD31) antibody, following the application of magnetic beads pre-coated with secondary antibody.
Categories
Primary Cells
Species
Rat
Applications
For research use only
Shipping
Dry Ice
Storage
Liquid Nitrogen
Quality Control
All cells test negatIVe for mycoplasma, bacteria, yeast, and fungi.
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