Zinc-fingers and homeobox (ZHX) proteins are transcription factors that interact with the activation domain of the A subunit of nuclear factor-Y (NF-YA). ZHX1-3 are ubiquitously expressed proteins expressed in various tissues. They act as transcriptional repressors and localize to the nucleus. The ZHX proteins contain two Cys2-His2-type zinc-finger motifs and five homeodomains (HDs). These domains allow the ZHX proteins to form homodimers, but they can also form heterodimers with each other. However, this dimerization is not required for repressor activity. Hypermethylation-mediated silencing of ZHX2 is an epigenetic event involved in hepatocellular carcinoma (HCC).
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Recombinant full-length human ZHX3 protein was used as the immunogen for the ZHX3 antibody.
Isotype
Mouse IgG2b
Species Reactivity
Human
Note
Optimal dilution of the ZHX3 antibody should be determined by the researcher.
Uniprot
Q9H4I2
Format
Purified
Purity
Protein A/G affinity
Storage
Aliquot the ZHX3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Applications
WB, ELISA, IP, FACS
Description
ELISA (order BSA-free format for coating),Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate),Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells