ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene.ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Rabbit
Immunogen Region
A portion of amino acids 247-382 from the human protein was used as the immunogen for the recombinant ZAP70 antibody.
Isotype
Rabbit IgG
Species Reactivity
Human
Note
Optimal dilution of the recombinant ZAP70 antibody should be determined by the researcher.
Uniprot
P43403
Format
Purified
Purity
Protein A affinity chromatography
Storage
Store the recombinant ZAP70 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
ELISA, IHC-P
Description
ELISA: 2-4ug/ml for coating (order Ab without BSA),Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT