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Home / Primary Antibodies

Vinculin Antibody (R30874)

SKU : BHA17104416

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Mfr.No.	R30874
Clonality	Polyclonal
Description	In mammalian cells, Vinculin is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. By linkage studies in a 3-generation family, Mulligan et al.(1992) mapped the VCL gene to chromosome 10q22.1-q23, distal to D10S22. It is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is though to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Multiple alteatively-spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. The human protein exhibits a greater-than-95% sequence identity to the chicken protein. Vinculin is a 117-kDa cytoskeletal protein with 1066 amino acids. The protein contains an acidic N-terminal domain and a basic C-terminal domain separated by a proline-rich middle segment. It has a globular head domain that contains binding sites for talin and alpha-actinin as well as a tyrosine phosphorylation site, while the tail region contains binding sites for F-actin, paxillin, and lipids.
Formulation	0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host	Rabbit
Immunogen Region	Amino acids 173-188 (KMIDERQQELTHQEHR) were used as the immunogen for this Vinculin antibody (100% homologous in human, mouse and rat).
Isotype	IgG
Predicted Reactivity	Mouse
Reactivity	Mouse
Recombinant	No
Subcellular Location	Cytoplasmic and cell membrane

Uniprot	P18206

Format	Antigen affinity purified
Purification	Antigen affinity
Storage	After reconstitution, the Vinculin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Applications	WB, IHC-P
Dilution	Weste blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml

Product Manual	Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17104416	R30874	100 ug	$419		Add to Cart

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