Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A portion of amino acids 2-466 from the human protein was used as the immunogen for the recombinant VIM antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human (broad species reactivity predicted)
Note
Optimal dilution of the recombinant VIM antibody should be determined by the researcher.
Uniprot
P08670
Format
Purified
Purity
Protein A/G affinity
Storage
Aliquot the recombinant VIM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.