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Home / Antibodies

Vimentin Antibody (V3935SAF)

SKU : BHA17125747

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NSJ Bioreagents

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Mfr.No.	V3935SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	This antibody specifically reacts with an ~ 58kDa protein identified as Vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Vimentin antibody alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for tumor sub-classification. Expression of vimentin, when used in conjunction with keratin antibody, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with vimentin antibody. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity with vimentin antibody is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Vimentin antibody is also useful as a tissue process control reagent.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	Recombinant protein was used as the immunogen for the Vimentin antibody.
Isotype	Mouse IgG1, kappa
Species Reactivity	Human
Note	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Uniprot	P08670

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the Vimentin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	WB, FACS, IHC-P
Description	Flow cytometry: 1-2ug/million cells,Weste blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17125747	V3935SAF-100UG	100 ug	$499		Add to Cart

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