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Home / Antibodies

VIM Antibody

SKU : BHA10559084

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA573495
Alternative Names	CTRCT30
Categories	Primary Antibodies
Clonality	polyclonal
Description	Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Sommers C.L., Walker-Jones D., Heckford S.E., Worland P., Valverius E., Clark R., McCormick F., Cancer Res. 49:4258-4263(1989) Kang S.-M., Shin M.-J., Kim J.-H., Oh J.-W. Proteomics 5:2227-2237(2005)Ahmed B.A., Bukhari I.A., Jeffus B.C., Harney J.T., Thyparambil S., Ziu E., Fraer M., Rusch N.J. PLoS ONE 4:E4730-E4730(2009)
Host	Rabbit
Immunogen	Peptide sequence around aa.452~456(G-Q-V-I-N) derived from Human Vimentin.
Involvement In Disease	Cataract 30, multiple types (CTRCT30)
Raised In	Rabbit
Reactivity	Monkey, Human, Mouse, Rat
Regulatory	RUO
Relevance	Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Sommers C.L., Walker-Jones D., Heckford S.E., Worland P., Valverius E., Clark R., McCormick F., Cancer Res. 49:4258-4263(1989) Kang S.-M., Shin M.-J., Kim J.-H., Oh J.-W. Proteomics 5:2227-2237(2005) Ahmed B.A., Bukhari I.A., Jeffus B.C., Harney J.T., Thyparambil S., Ziu E., Fraer M., Rusch N.J. PLoS ONE 4:E4730-E4730(2009)
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous level of total Vimentin protein.
Subcellular Location	Cytoplasm

Uniprot	P08670
Gene Id	7431
Database Links	HGNC:12692 OMIM:116300 KEGG:hsa:7431 STRING:9606.ENSP00000224237 UniGene:Hs.455493
Function	Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Protein Families	Intermediate filament family
Tissue Specificity	Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Weight	53,652 Da

Buffer	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Purity	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB, IF

Description

ELISA ; WB ; IF, WB ; ELISA ; IF

Dilution

WB	1:500-1:1000
IF	1:100-1:200

Tested Application

ELISA,WB,IF;WB:1:500-1:1000,IF:1:100-1:200

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10559084	CSB-PA573495	100ul	$297		Add to Cart

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