Rabbit Anti-Human VEGF Polyclonal

Mfr.No.	SPC-1291, SPC-1291D, SPC-1291D-A390, SPC-1291D-A488, SPC-1291D-A594, SPC-1291D-BI, SPC-1291D-FITC
Immunogen Species	Human
Alternative Names	Vascular Endothelial Growth Factor Antibody, Vascular Permeability Factor Antibody, VEGF-A Antibody, VEGFA Antibody, VEGFA_HUMAN Antibody, VPF Antibody
Categories	Antibodies
Cellular Localization	Secreted
Certificate Of Analysis	A 1:1000 dilution of SPC-1291 was sufficient for detection of VEGF in 10 ug of A431 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Description	Rabbit Anti-Human VEGF Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	Synthetic peptide corresponding to a region derived human vascular endothelial growth factor A, Homo Sapiens (Human)
Immunogen Region	Synthetic peptide corresponding to a region derived human vascular endothelial growth factor A
Isotype	IgG
Launch Date	2016/09/06
Net Weight (g)	0.1
Reactivity	Rat, Mouse, Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Mouse, Rat, Human
Tissue Specificity	Widely expressed (depending on isoform)

Swissprot	P15692
Accession Number	NP_003367.4
Gene Id	7422
Target	VEGF
Research Area	Cardiovascular System, Angiogenesis, Cardiovascular Growth Factors, VEGF, Cancer, Invasion/microenvironment, Cell Signaling, Metabolism, Metabolism processes, Hypoxia

Buffer	PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Dilutions	WB (1:1000), IHC (1:200); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Purity	Widely expressed (depending on isoform)
Scientific Background	Vascular endothelial growth factor (VEGF), is a dimeric glycoprotein with structural homology to PDGF. Several variants of VEGF have been described that arise by alternative mRNA splicing. VEGF stimulates cellular responses by binding to tyrosine kinase receptors on the cell surface. VEGF mRNA is formed in some primary tumors, VEGF is produced by tumor cell lines in vitro and VEGF mitogenic activity appears to be restricted to endothelial cells. A member of the PDGF receptor family, Flt, has been identified as a high-affinity receptor for VEGF.
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~24kDa and ~45kDa, endogenous level of total VEGFA protein.
Storage	-20ºC
Storage Buffer	PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	IHC, WB
Description	WB ; IHC
Dilution	WB (1:1000), IHC (1:200); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. de Vries C., et al. (1992) Science. 255(5047): 989991. 2. https://en.wikipedia.org/wiki/Vascular_endothelial_growth_factor

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11917442-1	SPC-1291D	Unconjugated	100 ul	$286	Add to Cart
BHA11917442-2	SPC-1291D-A390	ATTO 390	100 ul	$340	Add to Cart
BHA11917442-3	SPC-1291D-A488	ATTO 488	100 ul	$339	Add to Cart
BHA11917442-4	SPC-1291D-A594	ATTO 594	100 ul	$339	Add to Cart
BHA11917442-5	SPC-1291D-BI	Biotin	100 ul	$334	Add to Cart
BHA11917442-6	SPC-1291D-FITC	FITC	100 ul	$330	Add to Cart

VEGF Antibody - SPC-1291