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Home / Primary Antibodies

VCAM-1 Antibody (V2922SAF)

SKU : BHA17124513

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NSJ Bioreagents

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Mfr.No.	V2922SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	Recognizes a protein of 110kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1), L1CAM and INCAM-100). It is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. VCAM-1 serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	Stimulated human umbilical vein endothelial cells (HUVEC) were used as the immunogen for the VCAM-1 antibody.
Isotype	Mouse IgG1, kappa
Species Reactivity	Human
Note	Optimal dilution of the VCAM-1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	P19320

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the VCAM-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	FACS, IF, IHC-P
Description	Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17124513	V2922SAF-100UG	100 ug	$499		Add to Cart

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