Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.Adler A.J., Bioorg. Med. Chem. 9:1967-1976(2001).Sugano S., Nat. Genet. 36:40-45(2004).Venter J.C., Submitted (JUL-2005).
Host
Rabbit
Immunogen
Synthesized non-phosphopeptide derived from Human TUBA1/3/4 around the phosphorylation site of tyrosine 272 (A-T-Y(p)-A-P).
Involvement In Disease
Lissencephaly 3 (LIS3)
Raised In
Rabbit
Reactivity
Human, Mouse
Regulatory
RUO
Relevance
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Pathway
Gap junction Apoptosis
Protein Families
Tubulin family
Tissue Specificity
Expressed at a high level in fetal brain.
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Applications
ELISA, WB
Description
ELISA ; WB, WB ; ELISA
Dilution
WB
1:500-1:3000
Tested Application
ELISA,WB;WB:1:500-1:3000
Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Reviews of TUBA1A/TUBA1B/TUBA1C/TUBA3C/TUBA3E/TUBA4A (Ab-272) Antibody