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Home / Antibodies

Topoisomerase II alpha Antibody / TOP2A (V7234SAF)

SKU : BHA17126166

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NSJ Bioreagents

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Mfr.No.	V7234SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	This mAb recognizes a 170kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	Human recombinant partial protein (amino acids 1352-1493) was used as the immunogen for this Topoisomerase II alpha antibody.
Isotype	Mouse IgG2b, kappa
Species Reactivity	Human
Note	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Topoisomerase II alpha antibody to be titered up or down for optimal performance.1. Staining of FFPE tissue requires boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the Topoisomerase II alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	WB, IF, IHC-P
Description	Immunofluorescence: 1-2ug/ml,Weste blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml (1)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17126166	V7234SAF-100UG	100 ug	$499		Add to Cart

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