This mAb recognizes an ~170kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Amino acids 1352-1493 from the human protein were used as the immunogen for this TOP2A antibody.
Isotype
Mouse IgG2b, kappa
Species Reactivity
Human
Note
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TOP2A antibody to be titered up or down for optimal performance.
Uniprot
P11388
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the TOP2A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
WB, IF, IHC-P
Description
Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT