Terminal deoxynucleotidyltransferase (TdT) is a DNA polymerase which catalyzes the addition of deoxyribonucleotides onto the 3 termini of DNA. The human TdT gene maps to chromosome 10q24.1 and encodes a 510 amino acid protein. Human TdT is synthesized as a single chain peptide that elicits a minor preference for incorporation of deoxyribonucleotides over ribonucleotides forming DNA strands. TdT is present in immature thymocytes, some bone marrow cells, transformed pre-B and pre-T cell lines, and leukemia cells.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A portion of amino acids 52-192 from the human protein was used as the immunogen for the TdT antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human
Note
Optimal dilution of the TdT antibody should be determined by the researcher.
Uniprot
P04053
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the TdT antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
ELISA, IHC-P
Description
ELISA: 2-4ug/ml for coating; order Ab without BSA),Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT