Recognizes a protein of 71kDa, identified as Transcription Factor 4 (TCF4). It is a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A portion of amino acids 365-671 from the human protein was used as the immunogen for this TCF4 antibody.
Isotype
Mouse IgG2a, kappa
Species Reactivity
Human
Note
Optimal dilution of the TCF4 antibody should be determined by the researcher.
Uniprot
P15884
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the TCF4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
WB, ELISA, IHC-P
Description
ELISA: 2-4ug/ml (order BSA/azide-free format),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT