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Home / Primary Antibodies / Polyclonal Antibodies

SOD1 (UbetaB) Antibody - SPC-205

BHA11907431
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Email
[email protected]
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Swissprot P00441
Accession Number CAG46542
Gene Id 6647
Target SOD1
Research Area Cancer, Oxidative Stress, Neurodegeneration, ALS Disease, Neuroscience
Buffer PBS, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification Peptide Affinity purified
Scientific Background Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (2). It works by catalyzing the dismutation of the superoxide radical O2? to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (1,4). In general, SODs play a major role in antioxidant defense mechanisms (3). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (2). Misfolding of SOD1 has been implicated in Amyotrophic lateral sclerosis (ALS). Therefore conformation specific antibodies such as Anti-SOD1 (UbetaB), which targets an unfolded region of the beta barrel of SOD1, are useful for determining the conformation of SOD1 in affected tissues (5). This antibody can be used in conjunction with Anti-SOD1 (EDI) (SPC-206D) which detects an exposed dimer interface (EDI) of SOD1.
Shipping Temperature Blue Ice or 4°C
Specificity Recognizes a conformation specific epitope where the beta barrel is unfolded.
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications ELISA, WB, ICC/IF
Description WB ; ICC ; IF ; ELISA
Dilution WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180.

2. Furukawa Y., and O'Halloran T. (2006) Antioxid Redox Signal. 8(5-6):847-67.

3. Gao B., et al. (2003) Am J Physiol Lung Cell Mol Physiol. 284:L917-L925.

4. Hassan H.M. (1988) Free Radical Biol. Med. 5:377-385.

5. Kerman A., et al. (2010) Acta Neuropathol. 119:335-344.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11907431-1 SPC-205D Unconjugated 100 ug $306
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BHA11907431-2 SPC-205D-A390 ATTO 390 100 ug $360
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BHA11907431-3 SPC-205D-A488 ATTO 488 100 ug $359
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BHA11907431-4 SPC-205D-A594 ATTO 594 100 ug $359
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BHA11907431-5 SPC-205D-ALP Alkaline Phosphatase 100 ug $352
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BHA11907431-6 SPC-205D-APC APC 100 ug $358
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BHA11907431-7 SPC-205D-BI Biotin 100 ug $354
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BHA11907431-8 SPC-205D-FITC FITC 100 ug $350
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BHA11907431-9 SPC-205D-HRP HRP 100 ug $345
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BHA11907431-10 SPC-205D-PCP PerCP 100 ug $358
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BHA11907431-11 SPC-205D-RPE RPE 100 ug $355
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BHA11907431-12 SPC-205S Unconjugated 12 ug $44
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