Rabbit Anti-Human SOD1 (EDI) Polyclonal

Mfr.No.	SPC-206, SPC-206D, SPC-206D-A390, SPC-206D-A488, SPC-206D-A594, SPC-206D-ALP, SPC-206D-APC, SPC-206D-BI, SPC-206D-FITC, SPC-206D-HRP, SPC-206D-PCP, SPC-206D-RPE, SPC-206S
Immunogen Species	Human
Alternative Names	SEDI Antibody, SOD1 EDI Antibody, Superoxide dismutase 1 Antibody, SOD Antibody, SOD1 exposed dimer interface Antibody
Categories	Antibodies
Cellular Localization	Cytoplasm
Certificate Of Analysis	1ug/ml of SPC-206 was sufficient for detection of the exposed dimer interface of SOD1 by colorimetric dot blot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Description	Rabbit Anti-Human SOD1 (EDI) Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	N-terminal region of SOD1, exposed dimer interface (EDI), Homo Sapiens (Human)
Immunogen Region	C-terminal region of human SOD1, exposed dimer interface (EDI)
Launch Date	2013/09/25
Net Weight (g)	0.1
Reactivity	Rat, Mouse, Human, Pig
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Mouse, Rat, Pig, Human

Swissprot	P00441
Accession Number	CAG46542
Gene Id	6647
Target	SOD1
Research Area	Cancer, Oxidative Stress, Neurodegeneration, ALS Disease, Neuroscience

Buffer	PBS, 50% glycerol, 0.09% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:1000); optimal dilutions for assays should be determined by the user.
Purification	Protein A Purified
Scientific Background	Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (2). It works by catalyzing the dismutation of the superoxide radical O2? to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (1,4). In general, SODs play a major role in antioxidant defense mechanisms (3). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (2). Misfolding of SOD1 has been implicated in Amyotrophic lateral sclerosis (ALS). Therefore conformation specific antibodies such as SOD1 (EDI), which targets an exposed region of the dimer interface (EDI) of SOD1, are useful for determining the conformation of SOD1 in affected tissues (5). This antibody can be used in conjunction with SOD1 (UbetaB) (SPC-205D) which detects an unfolded beta barrel (UbetaB) of SOD1.
Shipping Temperature	Blue Ice or 4°C
Specificity	Recognizes a conformation specific epitope where the dimer interface is exposed.
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	ELISA, IHC, WB
Description	WB ; IHC ; ELISA
Dilution	WB (1:1000); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180. 2. Furukawa Y., and O'Halloran T. (2006) Antioxid Redox Signal. 8(5-6):847-67. 3. Gao B., et al. (2003) Am J Physiol Lung Cell Mol Physiol. 284:L917-L925. 4. Hassan H.M. (1988) Free Radical Biol. Med. 5:377-385. 5. Kerman A., et al. (2010) Acta Neuropathol. 119:335-344.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11907450-1	SPC-206D	Unconjugated	100 ug	$306	Add to Cart
BHA11907450-2	SPC-206D-A390	ATTO 390	100 ug	$360	Add to Cart
BHA11907450-3	SPC-206D-A488	ATTO 488	100 ug	$359	Add to Cart
BHA11907450-4	SPC-206D-A594	ATTO 594	100 ug	$359	Add to Cart
BHA11907450-5	SPC-206D-ALP	Alkaline Phosphatase	100 ug	$352	Add to Cart
BHA11907450-6	SPC-206D-APC	APC	100 ug	$358	Add to Cart
BHA11907450-7	SPC-206D-BI	Biotin	100 ug	$354	Add to Cart
BHA11907450-8	SPC-206D-FITC	FITC	100 ug	$350	Add to Cart
BHA11907450-9	SPC-206D-HRP	HRP	100 ug	$345	Add to Cart
BHA11907450-10	SPC-206D-PCP	PerCP	100 ug	$358	Add to Cart
BHA11907450-11	SPC-206D-RPE	RPE	100 ug	$355	Add to Cart
BHA11907450-12	SPC-206S	Unconjugated	12 ug	$44	Add to Cart

SOD1 (EDI) Antibody - SPC-206