JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.
Loding Loading ....
Home / Primary Antibodies / Polyclonal Antibodies

SOD (Mn) Antibody - SPC-117

BHA11906503
Contact us to order
Tel
+1 866.986.9598
Email
[email protected]
Get a Quote
Request More Infos
Order Now

Credit card payments now incur a 3% fee.

Contact us to order
Tel
+1 866.986.9598
Email
[email protected]
Get a Quote
Ask Merchant
Request More Infos
Sample Request
Swissprot P07895
Accession Number NP_058747.1
Gene Id 24787
Target SOD2
Research Area Cancer, Oxidative Stress, Cell Signaling, Neurodegeneration, Cardiovascular System, Neuroscience
Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:5000), IHC (1:100), ICC/IF (1:120); optimal dilutions for assays should be determined by the user.
Purification Protein A Purified
Scientific Background Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2? to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4).
There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~25kDa.
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications ELISA, IHC, IP, WB, ICC/IF
Description WB ; IHC ; ICC ; IF ; IP ; ELISA
Dilution WB (1:5000), IHC (1:100), ICC/IF (1:120); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Adachi T., et al. (1992). Clin. Chim. Acta. 212: 89-102.

2. Barrister J.V., et al. (1987). Crit. Rev. Biochem. 22:111-180.

3. Furukawa Y., O'Halloran T. (2006). Antioxidants & Redo Signaling. Vol 8, No 5,6.

4. Gao B., et al. (2003). Am J Physiol Lung Cell Mol Physiol 284: L917-L925.

5. Hassan H.M. (1988). Free Radical Biol. Med. 5: 377-385.

6. Kurobe N., et al. (1990) Biomedical Research. 11: 187-194

7. Wispe J.R., et al. (1989) BBA. 994: 30-36.

8. Xiao-Hong Liu., et al. (1993) Brain Research. 625: 29-37.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11906503-1 SPC-117D Unconjugated 100 ug $345
Add to Cart
BHA11906503-2 SPC-117C Unconjugated 25 ug $171
Add to Cart
BHA11906503-3 SPC-117D-A390 ATTO 390 100 ug $399
Add to Cart
BHA11906503-4 SPC-117D-A488 ATTO 488 100 ug $398
Add to Cart
BHA11906503-5 SPC-117D-A594 ATTO 594 100 ug $398
Add to Cart
BHA11906503-6 SPC-117D-ALP Alkaline Phosphatase 100 ug $392
Add to Cart
BHA11906503-7 SPC-117D-APC APC 100 ug $397
Add to Cart
BHA11906503-8 SPC-117D-BI Biotin 100 ug $394
Add to Cart
BHA11906503-9 SPC-117D-FITC FITC 100 ug $389
Add to Cart
BHA11906503-10 SPC-117D-HRP HRP 100 ug $385
Add to Cart
BHA11906503-11 SPC-117D-PCP PerCP 100 ug $397
Add to Cart
BHA11906503-12 SPC-117D-RPE RPE 100 ug $395
Add to Cart
BHA11906503-13 SPC-117S Unconjugated 12 ug $44
Add to Cart