SPF30 (survival of motor neuron-related-splicing factor 30), also known as SMNDC1 (survival motor neuron domain containing 1) or SMNR (SMN-related protein), is an essential splicing factor required for spliceosome assembly that belongs to the SMN family. It contains one Tudor domain with significant similarity to SMN (survival motor neuron) and is expressed in skeletal muscle, pancreas and heart, localizing to Cajal bodies and nuclear speckles. SPF30 plays an important role in spliceosome assembly and directly interacts with five U snRNPs. The loss of SPF30 causes spliceosome assembly to arrest at prespliceosomes (A complex). This supports a function for SPF30 in mediating the incorporation/recruitment of U4/U5/U6 tri-snRNP to the prespliceosome. In addition, the overexpression of SPF30 can lead to apoptosis.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Recombinant full-length human SMNDC1 protein was used as the immunogen for the SMNDC1 antibody.
Isotype
Mouse IgG2b
Species Reactivity
Human
Note
Optimal dilution of the SMNDC1 antibody should be determined by the researcher.
Uniprot
O75940
Format
Purified
Purity
Protein A/G affinity
Storage
Aliquot the SMNDC1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.