SM-MHC, or Myosin-11, is a smooth muscle myosin belonging to the myosin heavy chain family. Myosin-11 is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It is a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP. (Wiki)
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Human uterus extract was used as the immunogen for the SM-MHC antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human, Rat
Note
Optimal dilution of the SM-MHC antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Uniprot
P35749
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the SM-MHC antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
FACS, IF, IHC-P
Description
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT